Journal: Cell Death & Disease

Article Title: CDC42EP3 promotes glioma progression via regulation of CCND1

doi: 10.1038/s41419-022-04733-9

Figure Lengend Snippet: A Representative photographs of human glioma tissues and normal brain tissues after immunohistochemistry staining of CDC42EP3. The scale bar is 50 µm long. B Glioma patients with high expression of CDC42EP3 undergoes relatively shorter overall survival in comparison with those with low CDC42EP3 expression. C The relative mRNA levels of CDC42EP3 in SHG-44 and U251 cells and differences between shCtrl- and shCDC42EP3-harboring cells detected by qPCR and WB. Data were presented by mean with SD ( n ≥ 3). ** P < 0.01, *** P < 0.001.

Article Snippet: The gene chip analysis was undergone with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, USA) and then scanned by Affymetrix Scanner 3000 (Affymetrix, USA).

Techniques: Immunohistochemistry, Staining, Expressing, Comparison

Journal: Cell Death & Disease

Article Title: CDC42EP3 promotes glioma progression via regulation of CCND1

doi: 10.1038/s41419-022-04733-9

Figure Lengend Snippet: A The volcano plot of differentially expressed genes (DEGs) profiling between shCtrl- and shCDC42EP3-harboring SHG-44 cells. Red/green dots represents DEGs with the threshold of the absolute fold change >1.3 and the FDR <0.05. B CDC42EP3-related interaction network and signaling pathways identified by IPA in SHG-44 cells. C The changes in the mRNA expression of 20 DEGs following CDC42EP3 depletion in SHG-44 cells were identified by qPCR. All relative mRNA levels of the downstream genes of CDC42EP3 were significantly different between two groups (shCtrl vs. shCDC42EP3). D The changes in the expression of several downstream targets following CDC42EP3 depletion in SHG-44 cells identified by WB. E The differential expression of CCND1 in glioma tissues and normal brain tissues was analyzed based on the data collected from TCGA database. F ROC curve analyses and AUC values for CCND1 in glioma and normal brain tissues. Data were presented by mean with SD ( n ≥ 3). ** P < 0.01.

Article Snippet: The gene chip analysis was undergone with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, USA) and then scanned by Affymetrix Scanner 3000 (Affymetrix, USA).

Techniques: Protein-Protein interactions, Expressing, Quantitative Proteomics